Statistics Tutorial - Instrumental Analysis and Calibration

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This tutorial introduces the concepts and practises of statistics in Analytical Chemistry, by presenting problems in data analysis and demonstrating the steps to solve them. It is modular, so that you can either start at the beginning and continue sequentially, or skip material you are already familiar with. The tutorial includes the use of Microsoft Excel™ to perform the various operations, and is illustrated with numerous screen captures and problem-solving assignments.

We begin with an introduction to using Microsoft Excel™ for basic calculations and producing proper graphs from experimental data. We then review basic statistical concepts, such as the mean and standard deviation before discussing other important statistical concepts such as probability distributions.

We go on to describe the use of calibration curves and regression statistics, including how to use these to estimate limits of detection and the uncertainty in experimentally determined concentrations. Finally, the use of t-tests and F-tests for data comparison and evaluation is discussed.

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