@article {90,
	title = {Mass spectrometry methods to study protein-metabolite interactions.},
	journal = {Expert Opin Drug Discov},
	volume = {12},
	year = {2017},
	month = {2017 12},
	pages = {1271-1280},
	abstract = {<p><strong>INTRODUCTION: </strong>To ​​​​understand and manipulate biochemical processes and signaling pathways, the knowledge of endogenous protein-metabolite interactions would be extremely helpful. Recent developments in precision mass spectrometry, high-throughput proteomics and sensitive metabolomic profiling are beginning to converge on a possible solution, heralding a new era of global metabolome-proteome {\textquoteright}interactome{\textquoteright} studies that promise to change biomedical research and drug discovery. Areas covered: Here, we review innovative mass spectrometry-based methods and recent pioneering studies aimed at elucidating the physical associations of small molecule ligands with cellular proteins. The technologies covered belong to two main categories: tag-based and tag-free methods. We emphasize the latter in this review, and outline promising experimental workflows and key data analysis considerations involved. Expert opinion: Recent ground-breaking advances in chemical-proteomics technology and allied computational methods now make the global detection of protein-ligand engagement an increasingly attractive research problem. Despite ongoing challenges, rapid progress in the field is expected these coming next few years, leading to a refreshed systems biology research paradigm and much needed new opportunities for improving sparse drug discovery pipelines.</p>},
	keywords = {Biomedical Research, Drug Discovery, High-Throughput Screening Assays, Humans, Ligands, Mass Spectrometry, Metabolome, Metabolomics, Proteins, Proteome, Proteomics, Systems Biology},
	issn = {1746-045X},
	doi = {10.1080/17460441.2017.1378178},
	author = {Guo, Hongbo and Peng, Hui and Emili, Andrew}
}
