@article {297, title = {Binding of per- and polyfluoroalkyl substances with liver and serum proteins in rats: implications for physiologically based pharmacokinetic modelling}, journal = {Environment International}, volume = {201}, year = {2025}, author = {Fan, XR and Li, XM and Li, T and Shao, B and Niu, S and Fan, WH and Wang, Q and Zhong, L and Wang, XR and Wang, ZW and Ma, FJ and Zheng GM and Peng, H and Chen, LL and Dong, ZM} } @article {298, title = {Enantioselectivity in Metabolism and Toxicity of 6PPD-Quinone in Salmonids}, journal = {Environmental Science and Technology}, volume = {59}, year = {2025}, author = {Li, R and Barrett, H and Nair, P and Wang, MH and Tomlin, H and Atkinson, JB and Krogh, E and Xie, LN and Peng, H} } @article {227, title = {All-in-one digital microfluidics pipeline for proteomic sample preparation and analysis}, journal = {Chemical Science}, year = {2023}, chapter = {in press}, author = {Peng, JX and Chan, C and Zhang, SL and Sklavounos, AA and Olson, ME and Scott, EY and Hu, YC and Rajesh, V and LI, BB and Chamberlain, MD and Zhang, S and Peng, H and Wheeler, AR} } @article {225, title = {Building the Environmental Chemical-Protein Interaction Network (eCPIN): An Exposome-Wide Strategy for Bioactive Chemical Contaminant Identification}, journal = {Environmental Science and Technology}, year = {2023}, chapter = {in press}, author = {Gong, YF and Yang, DW and Barrett, H and Yang, DW and Peng, H} } @article {231, title = {Leaching of dibutyltin dichloride from virgin polyvinyl chloride (PVC) pipe material}, journal = {ACS ES\&T Water}, year = {2023}, month = {In press}, author = {Wu, MH and Chen, WZ and Peng, H and Almuhtaram H and Andrews RC} } @article {228, title = {Per- and Polyfluoroalkyl Substances in Canadian Fast Food Packaging}, journal = {Environ. Sci. Technol. Lett}, year = {2023}, author = {Schwartz-Narbonne, Heather and Xia, CJ and Shalin, A and Whitehead, HD and Yang, DW and Peaslee, Graham F and Wang, ZY and Wu, Y and Peng, H and Blum, A and Venier, M and Diamond, ML} } @article {232, title = {PPARg as a potential target for adipogenesis induced by fine particulate matter in 3T3-L1 preadipocytes}, journal = {Environmental Science and Technology}, year = {2023}, chapter = {in press}, author = {Cao, YQ and Chen, YY and Miao, K and Zhang, SY and Deng, FC and Zhu, M and Wang, C and Gu, W and Huang, YX and Shao, ZJ and Dong, XY and Gong YF and Peng, H and Yang, H and Wan, Y and Jia, XD and Tang, S} } @article {243, title = {Screening of Indoor Transformation Products of Organophosphates and Organophosphites with an in Silico Spectral Database}, journal = {ACS measurement Science Au (invited article)}, year = {2023}, chapter = {in press}, author = {Kutarna, S and Chen, WZ and Xiong, Y and Liu, RZ and Gong, YF and Peng, H} } @article {230, title = {Thiol Reactome: A Nontargeted Strategy to Precisely Identify Thiol Reactive Drinking Water Disinfection Byproducts}, journal = {Environmental Science and Technology}, year = {2023}, author = {Yeung, KY and Moore, N and Sun, JX and Edmonds, LT and Andrews, S and Hofmann, R and Peng, H} } @article {226, title = {Widespread Presence of Chlorinated Paraffins in Consumer Products}, journal = {Environmental Science: Processes \& Impacts}, year = {2023}, chapter = {in press}, author = {Kutarna, S and Du, X and Diamond, ML and Blum, A and Peng, H} } @article {199, title = {Community Surveillance of Omicron in Ontario: Wastewater-based Epidemiology Comes of Age}, journal = {Research Square}, year = {2022}, author = {Arts, E and Brown, S and Bulir, D and Charles, TC and DeGroot, CT and Delatolla, R and Desaulniers, JP and Edwards, EA and Fuzzen, M and Gilbride, K and Gilchrist, J and Goodridge, L and Graber, T and Habash, M and Juni, P and Kirkwood, A and Knockleby, J and Kyle, C and Landgraff, C and Mangat, C and Manuel, DG and McKay, RM and Mejia, E and Mloszewska, A and Ormeci, B and Oswald, C and Payne, SJ and Peng, H and Peterson, S and Poon, A and Servos, MR and Simmons, D and Sun, JX and Yang MQ and Ybazeta, G} } @article {202, title = {Early Warning Measurement of SARS-CoV-2 Variants of Concern in Wastewaters by Mass Spectrometry}, journal = {Environ. Sci. Technol. Lett}, year = {2022}, month = {06/2022}, author = {Peng, JX and Sun, JX and Yang, MQ and Gibson, R and Arts, E and Olabode, AS and Poon, AF and Wang, XY and Wheeler, AR and Edwards, E and Peng, H} } @article {198, title = {Quantitative Trend Analysis of SARS-CoV-2 RNA in Municipal Wastewater Exemplified with Sewershed-Specific COVID-19 Clinical Case Counts}, journal = {ACS ES\&T Water}, volume = {2}, year = {2022}, month = {05/2022}, author = {Pileggi, V and Shurgold, J and Sun, JX and Yang, MQ and Edwards, E and Peng, H and Tehrani A and Gilbride, KA and Oswald CJ and Wijayasri, S and AI-Bargash, D and Stuart, R and Khansari, Z and Raby, M and Thomas, J and Fletcher, T and Simon, A} } @article {178, title = {Quantitative Chemical Proteomics Reveals Interspecies Variations on Binding Schemes of L-FABP with Perfluorooctanesulfonate}, journal = {Environ. Sci. Technol.}, volume = {55}, year = {2021}, abstract = {

Evaluating interspecies toxicity variation is a long-standing challenge for chemical hazard assessment. This study developed a Quantitative Interspecies Thermal Shift Assay (QITSA) for in-situ, quantitative, and modest-throughput investigation of chemical-protein interactions in cell and tissue samples across species. By using liver fatty acid binding protein (L-FABP) as a case study, the QITSA method was benchmarked with six per- and polyfluoroalkyl substances (PFAS) and thermal shifts (∆Tm) were inversely related to their dissociation constants (R2=0.98). QITSA can also distinguish binding modes of chemicals exemplified by palmitic acid. The QITSA was applied to determine the interactions between perfluorooctanesulfonate(PFOS) and L-FABP in liver cells or tissues from human, mouse, rat, and zebrafish. The largest thermal stability enhancement by PFOS was observed for human L-FABP, followed by mouse, rat and zebrafish. While endogenous ligands were revealed to partially contribute to the large interspecies variation, recombinant proteins were employed to confirm the high binding affinity of PFOS to human L-FABP, compared to rat and mouse. This study implemented an experimental strategy to characterize chemical-protein interactions across species and future application of QITSA to other chemical contaminants is of great interest.

}, author = {Han, JJ and Fu, J and Sun, JX and Hall, DR and Yang, DW and Blatz, D and Houck, KA and Ng, CA and Doering, JA and LaLone, CA and Peng, H} } @article {175, title = {A Roadmap to the Structure-Related Metabolism Pathways of Per- and Polyfluoroalkyl Substances in the Early Life Stages of Zebrafish (Danio rerio)}, journal = {Environ. Health. Perspect.}, volume = {129}, year = {2021}, abstract = {

BACKGROUND: Thousands of per- and polyfluoroalkyl substances (PFAS) with diverse structures have been detected in the ambient environment. Apart from a few well studied PFAS, the structure-related toxicokinetics of a broader set of PFAS remain unclear.

OBJECTIVES: To understand the toxicokinetics of PFAS, we attempted to characterize the metabolism pathways of 74 structurally diverse PFAS samples from the U.S. EPA{\textquoteright}s PFAS screening library.

METHODS: Using the early life stages of zebrafish (Danio rerio) as a model, we determined the bioconcentration factors and phenotypic toxicities of 74 PFAS. Then, we applied High-Resolution Mass Spectrometry based non-targeted analysis to identify metabolites of PFAS in zebrafish larvae after five days of exposure, by incorporating retention time and mass spectra. In vitro enzymatic activity experiments with human recombinant liver carboxylesterase (hCES1) were employed to validate the structure-related hydrolysis of 11 selected PFASs.

RESULTS: Our findings identified five structural categories of PFAS prone to metabolism. The metabolism pathways of PFAS are highly related to their structures as exemplified by fluorotelomer alcohols that the predominance of β-oxidation or taurine conjugation pathways were primarily determined by the number of hydrocarbons. Hydrolysis is a major metabolism pathway for diverse PFAS and perfluoroalkyl carboxamides showed the highest in vivo hydrolysis rates, followed by carboxyesters and sulfonamides. The hydrolysis of PFAS was verified with recombinant hCES1, with strong substrate preferences towards perfluoroalkyl carboxamides.

CONCLUSIONS:We suggest that the roadmap of the structure-related metabolism pathways of PFAS established in this study would provide a starting point to inform the potential health risks of other PFAS.

}, author = {Han, JJ and Gu, W and Barrett, H and Yang, DW and Tang, S and Sun, JX and Liu, JB and Krause, HM and Houck, KA and Peng, H} } @article {141, title = {Monohaloacetic Acids and Monohaloacetamides Attack Distinct Cellular Proteome Thiols}, journal = {Environ. Sci. Technol.}, volume = {54}, year = {2020}, chapter = {in press}, author = {Hall, DR and Yeung, K and Peng, H} } @article {138, title = {Nontarget Screening of Per- and Polyfluoroalkyl Substances Binding to Human Liver Fatty Acid Binding Protein}, journal = {Environ. Sci. Technol.}, volume = {54}, year = {2020}, chapter = {5676-5686}, author = {Yang, DW and Han, JJ and Hall, DR and Sun, JX and Fu, J and Kutarna, S and Houck, KA and LaLone, CA and Doering, JA and Ng, CA and Peng, H} } @article {85, title = {Spatial and temporal trends in poly- and per-fluorinated compounds in the Laurentian Great Lakes Erie, Ontario and St. Clair.}, journal = {Environ Pollut}, volume = {237}, year = {2018}, month = {2018 Jun}, pages = {396-405}, abstract = {

The temporal and spatial trends in sediment of 22 poly- and perfluorinated (PFAS) compounds were investigated in the southern Great Lakes Erie and Ontario as well as Lake St. Clair. Surface concentrations measured by Ponar grab samples indicated a trend for greater concentrations near to urban sites. Mean concentrations ∑PFAS were 15.6, 18.2 and 19 ng g dm for Lakes St. Clair, Erie and Ontario, respectively. Perfluoro-n-butanoic acid (PFBA) and Perfluoro-n-hexanoic acid (PFHxA) were frequently determined in surface sediment and upper core samples indicating a shift in use patterns. Where PFBA was identified it was at relatively great concentrations typically \>10 ng g dm. However as PFBA and PFHxA are less likely to bind to sediment they may be indicative of pore water concentrations Sedimentation rates between Lake Erie and Lake Ontario differ greatly with greater rates observed in Lake Erie. In Lake Ontario, in general concentrations of PFAS observed in core samples closely follow the increase in use along with an observable change due to regulation implementation in the 1970s for water protection. However some of the more water soluble PFAS were observed in deeper core layers than the time of production could account for, indicating potential diffusion within the sediment. Given the greater sedimentation rates in Lake Erie, it was hoped to observe in greater resolution changes since the mid-1990s. However, though some decrease was observed at some locations the results are not clear. Many cores in Lake Erie had clearly observable gas voids, indicative of gas ebullition activity due to biogenic production, there were also observable mussel beds that could indicate mixing by bioturbation of core layers.

}, keywords = {Environmental Monitoring, Fluorocarbons, Great Lakes Region, Lakes, Ontario, Water Pollutants, Chemical, Water Pollution, Chemical}, issn = {1873-6424}, doi = {10.1016/j.envpol.2018.02.013}, author = {Codling, Garry and Sturchio, Neil C and Rockne, Karl J and Li, An and Peng, H and Tse, Timothy J and Jones, Paul D and Giesy, John P} }