@article {274, title = {Multigenerational effects of disperse blue 79 at environmentally relevant concentrations on zebrafish (Danio rerio) fecundity: An integrated approach.}, journal = {J Hazard Mater}, volume = {478}, year = {2024}, month = {2024 Oct 05}, pages = {135442}, abstract = {

The brominated azo dye (BAD) Disperse Blue (DB79) is a widespread environmental pollutant. The long-term toxicological effects of DB79 and the mechanisms thereof must be understood to allow assessment of the risks of DB79 pollution. A dual-omics approach employing in silico analysis, bioinformatics, and in vitro bioassays was used to investigate the transgenerational (F-F) toxicity of DB79 in zebrafish at environmentally relevant concentrations and identify molecular initiating events and key events associated with DB79-induced fertility disorders. Exposure to 500\ {\textmu}g/L DB79 decreased fecundity in the F and F generations by \>\ 30\ \% and increased the condition factor of the F generation 1.24-fold. PPARĪ±/RXR and PXR ligand binding activation were found to be critical molecular initiating events associated with the decrease in fecundity. Several key events (changes in fatty acid oxidation and uptake, lipoprotein metabolism, and xenobiotic metabolism and transport) involved in lipid dysregulation and xenobiotic disposition were found to be induced by DB79 through bioinformatic annotation using dual-omics data. The biomolecular underpinnings of decreased transgenerational fertility in zebrafish attributable to BAD exposure were elucidated and novel biomolecular targets in the adverse outcome pathway framework were identified. These results will inform future studies and facilitate the development of mitigation strategies.

}, keywords = {Animals, Azo Compounds, Female, Fertility, Male, Pregnane X Receptor, Water Pollutants, Chemical, Zebrafish}, issn = {1873-3336}, doi = {10.1016/j.jhazmat.2024.135442}, author = {Wang, Chao and Gu, Wen and Zhang, Shaoping and Li, Li and Kong, Jian and Zhi, Hong and Liu, Juan and Wang, Mengmeng and Miao, Ke and Li, Qi and Yu, Jie and Wang, Runming and He, Runming and Zhang, Shuyi and Deng, Fuchang and Duan, Shuling and Zhang, Qiannan and Liu, Zhenming and Yang, Hui and Jia, Xudong and Peng, Hui and Tang, Song} } @article {254, title = {Multiphase Ozonolysis of Bisphenol A: Chemical Transformations on Surfaces in the Environment.}, journal = {Environ Sci Technol}, year = {2024}, month = {2024 Feb 13}, abstract = {

High global plastic production volumes have led to the widespread presence of bisphenol compounds in human living and working environments. The most common bisphenol, bisphenol A (BPA), despite being endocrine disruptive and estrogenic, is still not fully banned worldwide, leading to continued human exposure via particles in air, dust, and surfaces in both outdoor and indoor environments. While its abundance is well documented, few studies have addressed the chemical transformations of BPA, the properties of its reactive products, and their toxicity. Here, the first gas-surface multiphase ozonolysis experiment of BPA thin films, at a constant ozone mixing ratio of 100 ppb, was performed in a flow tube for periods up to 24 h. Three transformation products involving the addition of 1, 2, and 3 oxygen atoms to the molecule were identified by LC-ESI-HRMS analyses. Exposure of indoor air to thin BPA surface films and BPA-containing thermal paper over periods of days validated the flow tube experiments, demonstrating the rapid nature of this multiphase ozonolysis reaction at atmospherically relevant ozone levels. Multiple transformation pathways are proposed that are likely applicable to not only BPA but also emerging commercial bisphenol products.

}, issn = {1520-5851}, doi = {10.1021/acs.est.3c08932}, author = {Yu, Jie and Gong, Yufeng and Nair, Pranav and Liggio, John and Peng, Hui* and Abbatt, Jonathan P D*} } @article {276, title = {Structurally Selective Ozonolysis of -Phenylenediamines and Toxicity in Coho Salmon and Rainbow Trout.}, journal = {Environ Sci Technol}, year = {2024}, month = {2024 Nov 23}, abstract = {

The tire-rubber-derived ozonation product of -(1,3-dimethylbutyl)-{\textquoteright}-phenyl--phenylenediamine (6PPD), -(1,3-dimethylbutyl)-{\textquoteright}-phenyl--phenylenediamine-quinone (6PPD-Q), was recently discovered to cause acute mortality in coho salmon (). -Phenylenediamines (PPDs) with variable side chains distinct from 6PPD have been identified as potential replacement antioxidants, but their toxicities remain unclear under environmentally relevant ozone conditions. We herein tested the multiphase gas-surface ozone reactivity of four select PPDs [6PPD, -isopropyl-{\textquoteright}-phenyl--phenylenediamine (IPPD), ,{\textquoteright}-diphenyl--phenylenediamine (DPPD), and -phenyl-{\textquoteright}-cyclohexyl--phenylenediamine (CPPD)] and evaluated the toxicity of their reaction mixtures in coho salmon, rainbow trout (), and fathead minnow (). 6PPD and IPPD were found to rapidly react with ozone, while no significant multiphase ozone reactivity was observed for DPPD or CPPD. The viability of coho salmon CSE-119 cells was strongly affected by the ozonolysis products of 6PPD but not by those of the other three PPDs. Acute mortality was only observed in juvenile rainbow trout that were exposed to oxidized 6PPD, suggesting a common mechanism of toxic action in the two salmonid fish species. This study reports the structurally selective ozone reactivity of PPDs and the unique toxicity of 6PPD ozonolysis mixtures, which demonstrates that other PPDs are potential alternative antioxidants.

}, issn = {1520-5851}, doi = {10.1021/acs.est.4c04817}, author = {Xie, Linna and Yu, Jie and Nair, Pranav and Sun, Jianxian and Barrett, Holly and Meek, Oliver and Qian, Xing and Yang, Diwen and Kennedy, Lisa and Kozakiewicz, Derek and Hao, Chunyan and Hansen, John D and Greer, Justin B and Abbatt, Jonathan P D and Peng, Hui} }