@article {101, title = {High Conservation in Transcriptomic and Proteomic Response of White Sturgeon to Equipotent Concentrations of 2,3,7,8-TCDD, PCB 77, and Benzo[a]pyrene.}, journal = {Environ Sci Technol}, volume = {50}, year = {2016}, month = {2016 05 03}, pages = {4826-35}, abstract = {

Adverse effects associated with exposure to dioxin-like compounds (DLCs) are mediated primarily through activation of the aryl hydrocarbon receptor (AHR). However, little is known about the cascades of events that link activation of the AHR to apical adverse effects. Therefore, this study used high-throughput, next-generation molecular tools to investigate similarities and differences in whole transcriptome and whole proteome responses to equipotent concentrations of three agonists of the AHR, 2,3,7,8-TCDD, PCB 77, and benzo[a]pyrene, in livers of a nonmodel fish, the white sturgeon (Acipenser transmontanus). A total of 926 and 658 unique transcripts were up- and down-regulated, respectively, by one or more of the three chemicals. Of the transcripts shared by responses to all three chemicals, 85\% of up-regulated transcripts and 75\% of down-regulated transcripts had the same magnitude of response. A total of 290 and 110 unique proteins were up- and down-regulated, respectively, by one or more of the three chemicals. Of the proteins shared by responses to all three chemicals, 70\% of up-regulated proteins and 48\% of down-regulated proteins had the same magnitude of response. Among treatments there was 68\% similarity between the global transcriptome and global proteome. Pathway analysis revealed that perturbed physiological processes were indistinguishable between equipotent concentrations of the three chemicals. The results of this study contribute toward more completely describing adverse outcome pathways associated with activation of the AHR.

}, keywords = {Animals, Benzo(a)pyrene, Dioxins, Fishes, Polychlorinated Dibenzodioxins, Proteomics}, issn = {1520-5851}, doi = {10.1021/acs.est.6b00490}, author = {Doering, Jon A and Tang, Song and Peng, Hui and Eisner, Bryanna K and Sun, Jianxian and Giesy, John P and Wiseman, Steve and Hecker, Markus} } @article {119, title = {Tissue distribution and maternal transfer of poly- and perfluorinated compounds in Chinese sturgeon (Acipenser sinensis): implications for reproductive risk.}, journal = {Environ Sci Technol}, volume = {44}, year = {2010}, month = {2010 Mar 01}, pages = {1868-74}, abstract = {

It is critical to investigate the tissue distribution and maternal transfer of poly- and perfluorinated compounds (PFCs) in wild fish for assessing potential effects on ecosystems. Concentrations of 23 PFCs in nine organs and egg were measured in 16 17- to 25-year-old female Chinese sturgeon (Acipenser sinensis, an anadromous fish), that died during propagation. Three polyfluorinated amides were detected in stomach, intestine, and gills and 7:3 FTCA was specifically accumulated in liver. The greatest total concentration of PFCs in egg was 35.1 +/- 10.4 ng/g ww and was predominated by perfluorooctane sulfonate (PFOS) and perfluorotridecanoate acid (PFTriDA). The longer-chain C(11)-C(14) and C(16) perfluorinated carboxylates were more accumulated in Chinese sturgeon than PFOS, partly due to the increasing trends of PFCAs with fish age. Maternal transfer ratios of PFCs expressed as ratios of concentrations in the egg to those in the liver ranged from 0.79 (perfluorooctanoate) to 5.5 (PFTriDA), depending on their carbon chain lengths or protein-water coefficients. The PFOS equivalent of PFC mixtures, calculated by multiplying the relative potency factor of each PFC to PFOS by the corresponding concentration, ranged from 90.6 to 262 ng/g. The hazard quotient was 0.20, implying potential reproductive effects of PFCs on Chinese sturgeon.

}, keywords = {Alkanesulfonic Acids, Animals, Female, Fertility, Fertilization in Vitro, Fishes, Fluorocarbons, Organ Specificity, Reproduction, Risk Factors, Tissue Distribution}, issn = {0013-936X}, doi = {10.1021/es903248d}, author = {Peng, Hui and Wei, Qiwei and Wan, Yi and Giesy, John P and Li, Luoxin and Hu, Jianying} } @article {120, title = {Malformations of the endangered Chinese sturgeon, Acipenser sinensis, and its causal agent.}, journal = {Proc Natl Acad Sci U S A}, volume = {106}, year = {2009}, month = {2009 Jun 09}, pages = {9339-44}, abstract = {

The anadromous Chinese sturgeon (Acipenser sinensis) is endangered and listed among the first class of protected animals in China. The possible causes for the decline of this species are the effects of synthetic chemicals, and loss of critical habitat. Chinese sturgeon in the Yangtze River have accumulated triphenyltin (TPT) to 31-128 ng/g wet weigh (ww) in liver, which is greater than the concentrations of tributyltin (\<1.0 ng/g ww). Maternal transfer of TPT has resulted in concentrations of 25.5 +/- 13.0 ng/g ww in eggs of wild Chinese sturgeon, which poses a significant risk to the larvae naturally fertilized or hatched in the Yangtze River. The incidence of deformities in fry was 7.5\%, with 1.2\% of individuals exhibiting ocular abnormal development, and 6.3\% exhibited skeletal/morphological deformations. The incidences of both ocular and skeletal/morphological deformations were directly proportional to the TPT concentration in the eggs of both the Chinese sturgeon and the Siberian sturgeon (Acipenser baerii) in controlled laboratory studies. The rates of deformities in the controlled studies were consistent with the rates caused at the similar concentrations in eggs collected from the field. Thus, TPT is the causal agent to induce the malformation of larvae of Chinese sturgeon. The incidence of deformed larvae of Chinese sturgeon is an indicator of overall population-level effects of TPT on Chinese sturgeon, because TPT at environmentally relevant concentrations can result in significantly decrease both quality and quantity of eggs and spawning frequency of fish.

}, keywords = {Animals, China, Fishes, Organotin Compounds, Trialkyltin Compounds, Water Pollutants, Chemical}, issn = {1091-6490}, doi = {10.1073/pnas.0809434106}, author = {Hu, Jianying and Zhang, Zhaobin and Wei, Qiwei and Zhen, Huajun and Zhao, Yanbin and Peng, Hui and Wan, Yi and Giesy, John P and Li, Luoxin and Zhang, Bo} }