@article {128,
	title = {Underlying mechanisms of reproductive toxicity caused by multigenerational exposure of 2, bromo-4, 6-dinitroaniline (BDNA) to Zebrafish (Danio rerio) at environmental relevant levels.},
	journal = {Aquat Toxicol},
	volume = {216},
	year = {2019},
	month = {2019 Nov},
	pages = {105285},
	abstract = {<p>2-bromo-4, 6-dinitroaniline (BDNA) is a mutagenic aromatic amine involved in the production and degradation of Disperse blue 79, one of the most extensively used brominated azo dyes. In our previous study, a multigenerational exposure of BDNA (0.5, 5, 50 and 500 μg/L) to zebrafish from F adult to F larvae including a recovery group in F larvae was conducted. The effects on apical points observed in individuals and the long-term effects predicted on population were all related to reproduction. In this study, we performed molecular analysis to elucidate the underlying mechanisms of the reproductive toxicity of BDNA. In F generation, measurement of vitellogenin and transcription levels of genes associated with hypothalamus-pituitary-gland (HPG) axis, estrogen receptor (ER) and androgen receptor (AR) were conducted. There was a decrease in VTG level in the blood of F female fish and transcription of genes related to ER was more affected than that of genes related to AR. These results were consistent with adverse effects that sexual differentiation was biased towards males and fecundity was impaired in a concentration-dependent manner in adults of F generation after 150 days exposure. In F generation, global gene transcriptions of F larvae were investigated. It was uncovered that processes related to apoptosis, development and DNA damage were strongly affected. Alterations to these biological pathways accounted for the irreversible parental influence on a significant decrease in hatchability and increase in abnormality of F larvae. All evidence suggested that the multigenerational exposure of BDNA posed lasting effects transmitted from parents to offspring that persisted after exposure ceased.</p>},
	keywords = {Aniline Compounds, Animals, DNA Damage, Environmental Exposure, Female, Larva, Male, Principal Component Analysis, Reproduction, Toxicity Tests, Transcription, Genetic, Transcriptome, Vitellogenins, Water Pollutants, Chemical, Zebrafish},
	issn = {1879-1514},
	doi = {10.1016/j.aquatox.2019.105285},
	author = {Xie, Xianyi and Jin, Yaru and Ma, Zhiyuan and Tang, Song and Peng, Hui and Giesy, John P and Liu, Hongling}
}
@article {119,
	title = {Tissue distribution and maternal transfer of poly- and perfluorinated compounds in Chinese sturgeon (Acipenser sinensis): implications for reproductive risk.},
	journal = {Environ Sci Technol},
	volume = {44},
	year = {2010},
	month = {2010 Mar 01},
	pages = {1868-74},
	abstract = {<p>It is critical to investigate the tissue distribution and maternal transfer of poly- and perfluorinated compounds (PFCs) in wild fish for assessing potential effects on ecosystems. Concentrations of 23 PFCs in nine organs and egg were measured in 16 17- to 25-year-old female Chinese sturgeon (Acipenser sinensis, an anadromous fish), that died during propagation. Three polyfluorinated amides were detected in stomach, intestine, and gills and 7:3 FTCA was specifically accumulated in liver. The greatest total concentration of PFCs in egg was 35.1 +/- 10.4 ng/g ww and was predominated by perfluorooctane sulfonate (PFOS) and perfluorotridecanoate acid (PFTriDA). The longer-chain C(11)-C(14) and C(16) perfluorinated carboxylates were more accumulated in Chinese sturgeon than PFOS, partly due to the increasing trends of PFCAs with fish age. Maternal transfer ratios of PFCs expressed as ratios of concentrations in the egg to those in the liver ranged from 0.79 (perfluorooctanoate) to 5.5 (PFTriDA), depending on their carbon chain lengths or protein-water coefficients. The PFOS equivalent of PFC mixtures, calculated by multiplying the relative potency factor of each PFC to PFOS by the corresponding concentration, ranged from 90.6 to 262 ng/g. The hazard quotient was 0.20, implying potential reproductive effects of PFCs on Chinese sturgeon.</p>},
	keywords = {Alkanesulfonic Acids, Animals, Female, Fertility, Fertilization in Vitro, Fishes, Fluorocarbons, Organ Specificity, Reproduction, Risk Factors, Tissue Distribution},
	issn = {0013-936X},
	doi = {10.1021/es903248d},
	author = {Peng, Hui and Wei, Qiwei and Wan, Yi and Giesy, John P and Li, Luoxin and Hu, Jianying}
}
