@article {274, title = {Multigenerational effects of disperse blue 79 at environmentally relevant concentrations on zebrafish (Danio rerio) fecundity: An integrated approach.}, journal = {J Hazard Mater}, volume = {478}, year = {2024}, month = {2024 Oct 05}, pages = {135442}, abstract = {

The brominated azo dye (BAD) Disperse Blue (DB79) is a widespread environmental pollutant. The long-term toxicological effects of DB79 and the mechanisms thereof must be understood to allow assessment of the risks of DB79 pollution. A dual-omics approach employing in silico analysis, bioinformatics, and in vitro bioassays was used to investigate the transgenerational (F-F) toxicity of DB79 in zebrafish at environmentally relevant concentrations and identify molecular initiating events and key events associated with DB79-induced fertility disorders. Exposure to 500\ {\textmu}g/L DB79 decreased fecundity in the F and F generations by \>\ 30\ \% and increased the condition factor of the F generation 1.24-fold. PPARα/RXR and PXR ligand binding activation were found to be critical molecular initiating events associated with the decrease in fecundity. Several key events (changes in fatty acid oxidation and uptake, lipoprotein metabolism, and xenobiotic metabolism and transport) involved in lipid dysregulation and xenobiotic disposition were found to be induced by DB79 through bioinformatic annotation using dual-omics data. The biomolecular underpinnings of decreased transgenerational fertility in zebrafish attributable to BAD exposure were elucidated and novel biomolecular targets in the adverse outcome pathway framework were identified. These results will inform future studies and facilitate the development of mitigation strategies.

}, keywords = {Animals, Azo Compounds, Female, Fertility, Male, Pregnane X Receptor, Water Pollutants, Chemical, Zebrafish}, issn = {1873-3336}, doi = {10.1016/j.jhazmat.2024.135442}, author = {Wang, Chao and Gu, Wen and Zhang, Shaoping and Li, Li and Kong, Jian and Zhi, Hong and Liu, Juan and Wang, Mengmeng and Miao, Ke and Li, Qi and Yu, Jie and Wang, Runming and He, Runming and Zhang, Shuyi and Deng, Fuchang and Duan, Shuling and Zhang, Qiannan and Liu, Zhenming and Yang, Hui and Jia, Xudong and Peng, Hui and Tang, Song} } @article {128, title = {Underlying mechanisms of reproductive toxicity caused by multigenerational exposure of 2, bromo-4, 6-dinitroaniline (BDNA) to Zebrafish (Danio rerio) at environmental relevant levels.}, journal = {Aquat Toxicol}, volume = {216}, year = {2019}, month = {2019 Nov}, pages = {105285}, abstract = {

2-bromo-4, 6-dinitroaniline (BDNA) is a mutagenic aromatic amine involved in the production and degradation of Disperse blue 79, one of the most extensively used brominated azo dyes. In our previous study, a multigenerational exposure of BDNA (0.5, 5, 50 and 500 μg/L) to zebrafish from F adult to F larvae including a recovery group in F larvae was conducted. The effects on apical points observed in individuals and the long-term effects predicted on population were all related to reproduction. In this study, we performed molecular analysis to elucidate the underlying mechanisms of the reproductive toxicity of BDNA. In F generation, measurement of vitellogenin and transcription levels of genes associated with hypothalamus-pituitary-gland (HPG) axis, estrogen receptor (ER) and androgen receptor (AR) were conducted. There was a decrease in VTG level in the blood of F female fish and transcription of genes related to ER was more affected than that of genes related to AR. These results were consistent with adverse effects that sexual differentiation was biased towards males and fecundity was impaired in a concentration-dependent manner in adults of F generation after 150 days exposure. In F generation, global gene transcriptions of F larvae were investigated. It was uncovered that processes related to apoptosis, development and DNA damage were strongly affected. Alterations to these biological pathways accounted for the irreversible parental influence on a significant decrease in hatchability and increase in abnormality of F larvae. All evidence suggested that the multigenerational exposure of BDNA posed lasting effects transmitted from parents to offspring that persisted after exposure ceased.

}, keywords = {Aniline Compounds, Animals, DNA Damage, Environmental Exposure, Female, Larva, Male, Principal Component Analysis, Reproduction, Toxicity Tests, Transcription, Genetic, Transcriptome, Vitellogenins, Water Pollutants, Chemical, Zebrafish}, issn = {1879-1514}, doi = {10.1016/j.aquatox.2019.105285}, author = {Xie, Xianyi and Jin, Yaru and Ma, Zhiyuan and Tang, Song and Peng, Hui and Giesy, John P and Liu, Hongling} } @article {106, title = {Levels of Phthalate Metabolites in Urine of Pregnant Women and Risk of Clinical Pregnancy Loss.}, journal = {Environ Sci Technol}, volume = {49}, year = {2015}, month = {2015 Sep 01}, pages = {10651-7}, abstract = {

Toxicological studies have shown that phthalate esters (PAEs), a class of widely used and environmentally prevalent chemicals, can increase the abortion rate in animals, but epidemiological evidence is scarce. This study aimed to explore the relationship between the urinary concentration of phthalate metabolites and the risk of clinical pregnancy loss. A total of 132 women who underwent clinical pregnancy loss (cases) and 172 healthy pregnant women (controls) were recruited from Beijing, China. Eight phthalate metabolites in urine were determined by ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Five phthalate metabolites, monomethyl phthalate (MMP), monoethyl phthalate (MEP), monoisobutyl phthalate (MiBP), mono-n-butyl phthalate (MnBP), and mono(2-ethlyhexyl) phthalate (MEHP), were detected in at least 95\% of the urine samples, with the highest median concentration of 51.0 μg/g of creatinine for MnBP of all participants. The differences in urinary concentrations of phthalate metabolites between cases and controls were evaluated using the Mann-Whitney U test. The concentrations of MEP (median of 18.7 μg/g of creatinine), MiBP (23.3 μg/g of creatinine), and MnBP (58.2 μg/g of creatinine) detected in the cases were significantly higher than those (15.7 μg/g of creatinine for MEP, 19.4 μg/g of creatinine for MiBP, and 43.9 μg/g of creatinine for MnBP) in the controls (p \< 0.05). Increasing risks of clinical pregnancy loss were observed from the first to fourth quartiles of the MEP, MiBP, and MnBP concentrations (p \< 0.05 for trend). We concluded that exposure to MEP, MiBP, and MnBP was associated with an increased risk of clinical pregnancy loss.

}, keywords = {Adult, Beijing, Case-Control Studies, Embryo Loss, Environmental Monitoring, Esters, Female, Humans, Metabolome, Middle Aged, Odds Ratio, Phthalic Acids, Pregnancy, Risk Factors, Young Adult}, issn = {1520-5851}, doi = {10.1021/acs.est.5b02617}, author = {Mu, Di and Gao, Fumei and Fan, Zhanlan and Shen, Huan and Peng, Hui and Hu, Jianying} } @article {113, title = {High-density lipoprotein of patients with type 2 diabetes mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate.}, journal = {Cardiovasc Diabetol}, volume = {12}, year = {2013}, month = {2013 Jan 30}, pages = {27}, abstract = {

BACKGROUND: Dysfunctional high-density lipoprotein (HDL) may have pro-inflammatory effects on the endothelial cells,which causes atherosclerosis in type 2 diabetes mellitus (T2DM). HDL is a major carrier of sphingosine-1-phosphate (S1P) in plasma while S1P exhibits multiple biological activities. However, potential role of HDL and S1P in T2DM remains unexplored. We hypothesized that diabetic HDL with higher contents of S1P exerts beneficial effects on the vascular system.

METHODS: Subjects with T2DM with or without proved large arteries atherosclerosis and normal controls (n=15 for each group) were recruited in the present study. HDL was isolated from the subjects by ultracentrifugation. The levels of HDL-associated S1P were determined by UPLC-MS/MS. The protective function of diabetic HDL and S1P was evaluated by measuring cyclooxygenase-2 (COX-2) expression and prostacyclin I-2 (PGI-2) release by human umbilical vein endothelial cells (HUVECs) using western blot and enzyme-linked immunosorbent assay (ELISA), respectively.

RESULTS: The S1P levels in isolated HDL were significantly increased in T2DM subjects compared with controls (235.6 {\textpm} 13.4 vs 195.0 {\textpm} 6.4 ng/mg, P\< 0.05). The diabetic HDL exerted greater protective effects on inducing COX-2 expression and PGI-2 release by HUVECs than those of control HDL (p \< 0.05, p \< 0.01, respectively). Pertussis toxin, a common inhibitor of G-couple protein receptors, and VPC 23019, an antagonist of S1P receptor 1 and 3 significantly attenuated HDL-induced COX-2 expression and PGI-2 release.

CONCLUSIONS: Diabetic HDL carries higher level of S1P compared with normal HDL, which has the potential to contribute to protective effects on endothelial cells by inducing COX-2 expression and PGI-2 release. These findings provide a new insight of S1P function in T2DM patients, possibly leading to a new therapeutic target.

}, keywords = {Adult, Aged, Cells, Cultured, Cyclooxygenase 2, Diabetes Mellitus, Type 2, Endothelial Cells, Epoprostenol, Female, Gene Expression Regulation, Enzymologic, Human Umbilical Vein Endothelial Cells, Humans, Lipoproteins, HDL, Lysophospholipids, Male, Middle Aged, Sphingosine, Up-Regulation}, issn = {1475-2840}, doi = {10.1186/1475-2840-12-27}, author = {Tong, Xunliang and Peng, Hui and Liu, Donghui and Ji, Liang and Niu, Chenguang and Ren, Jun and Pan, Bing and Hu, Jianying and Zheng, Lemin and Huang, Yining} } @article {115, title = {Tissue distribution, maternal transfer, and age-related accumulation of dechloranes in Chinese sturgeon.}, journal = {Environ Sci Technol}, volume = {46}, year = {2012}, month = {2012 Sep 18}, pages = {9907-13}, abstract = {

While dechloranes have been detected in environmental media and wildlife, limited information was available on their tissue distribution in wildlife. Syn-dechlorane plus (syn-DP), anti-DP, syn-undecachloropentacyclooctadecadiene (syn-Cl(11)DP), anti-Cl(11)DP, dechlorane 602, dechlorane 603 and mirex were measured in 13 organs of 17 female Chinese sturgeon (Acipenser sinensis). Dechloranes were detected in all tissues and the highest concentrations of total dechloranes were detected in heart (87 pg/g ww, 4.5-645 pg/g ww), followed by adipose (61 pg/g ww, 14-531 pg/g ww) and eggs (57 pg/g ww, 13-261 pg/g ww). The tissue distribution of DP was mainly determined by lipid partition, while Dec 602 and Dec 603 preferred to accumulate in the intestine and stomach. The values of f(anti) (the concentration of anti-DP relative to the sum concentration of DP) in maternal tissues (0.72 {\textpm} 0.03 in muscle) were significantly higher than those found in eggs (0.65 {\textpm} 0.04) (p \< 0.001), while f(anti-Cl11DP) (the concentration of anti-Cl(11)DP relative to the sum concentration of Cl(11)DP) in maternal tissues (0.59 {\textpm} 0.10 in muscle) was significantly lower than that in eggs (0.75 {\textpm} 0.13) (p \< 0.05). High maternal transfer efficiencies of dechloranes were observed in eggs, which accounted for 49\% of the total body burden, and the ratios of concentrations in eggs to maternal tissues (EMR) for mirex, Dec 602, Dec 603, syn-Cl(11)DP, anti-Cl(11)DP, syn-DP, and anti-DP were 18, 8.8, 5.2, 2.6, 5.2, 5.5 and 3.7, respectively, which are dependent on their K(OW) values (r = -0.66, p \< 0.01). Negative age-related trends were observed for mirex, Dec 603, syn-Cl(11)DP and anti-Cl(11)DP in eggs (R(2) = 0.28-0.38, p = 0.02-0.05), which were possibly due to their high transfer efficiencies to eggs from maternal body (49\%).

}, keywords = {Animals, China, Eggs, Environmental Monitoring, Female, Fishes, Flame Retardants, Hydrocarbons, Chlorinated, Polycyclic Compounds, Tissue Distribution, Water Pollutants, Chemical}, issn = {1520-5851}, doi = {10.1021/es3025879}, author = {Peng, Hui and Zhang, Kun and Wan, Yi and Hu, Jianying} } @article {119, title = {Tissue distribution and maternal transfer of poly- and perfluorinated compounds in Chinese sturgeon (Acipenser sinensis): implications for reproductive risk.}, journal = {Environ Sci Technol}, volume = {44}, year = {2010}, month = {2010 Mar 01}, pages = {1868-74}, abstract = {

It is critical to investigate the tissue distribution and maternal transfer of poly- and perfluorinated compounds (PFCs) in wild fish for assessing potential effects on ecosystems. Concentrations of 23 PFCs in nine organs and egg were measured in 16 17- to 25-year-old female Chinese sturgeon (Acipenser sinensis, an anadromous fish), that died during propagation. Three polyfluorinated amides were detected in stomach, intestine, and gills and 7:3 FTCA was specifically accumulated in liver. The greatest total concentration of PFCs in egg was 35.1 +/- 10.4 ng/g ww and was predominated by perfluorooctane sulfonate (PFOS) and perfluorotridecanoate acid (PFTriDA). The longer-chain C(11)-C(14) and C(16) perfluorinated carboxylates were more accumulated in Chinese sturgeon than PFOS, partly due to the increasing trends of PFCAs with fish age. Maternal transfer ratios of PFCs expressed as ratios of concentrations in the egg to those in the liver ranged from 0.79 (perfluorooctanoate) to 5.5 (PFTriDA), depending on their carbon chain lengths or protein-water coefficients. The PFOS equivalent of PFC mixtures, calculated by multiplying the relative potency factor of each PFC to PFOS by the corresponding concentration, ranged from 90.6 to 262 ng/g. The hazard quotient was 0.20, implying potential reproductive effects of PFCs on Chinese sturgeon.

}, keywords = {Alkanesulfonic Acids, Animals, Female, Fertility, Fertilization in Vitro, Fishes, Fluorocarbons, Organ Specificity, Reproduction, Risk Factors, Tissue Distribution}, issn = {0013-936X}, doi = {10.1021/es903248d}, author = {Peng, Hui and Wei, Qiwei and Wan, Yi and Giesy, John P and Li, Luoxin and Hu, Jianying} }