@article {274,
	title = {Multigenerational effects of disperse blue 79 at environmentally relevant concentrations on zebrafish (Danio rerio) fecundity: An integrated approach.},
	journal = {J Hazard Mater},
	volume = {478},
	year = {2024},
	month = {2024 Oct 05},
	pages = {135442},
	abstract = {<p>The brominated azo dye (BAD) Disperse Blue (DB79) is a widespread environmental pollutant. The long-term toxicological effects of DB79 and the mechanisms thereof must be understood to allow assessment of the risks of DB79 pollution. A dual-omics approach employing in silico analysis, bioinformatics, and in vitro bioassays was used to investigate the transgenerational (F-F) toxicity of DB79 in zebrafish at environmentally relevant concentrations and identify molecular initiating events and key events associated with DB79-induced fertility disorders. Exposure to 500\&nbsp;{\textmu}g/L DB79 decreased fecundity in the F and F generations by \&gt;\&nbsp;30\&nbsp;\% and increased the condition factor of the F generation 1.24-fold. PPARα/RXR and PXR ligand binding activation were found to be critical molecular initiating events associated with the decrease in fecundity. Several key events (changes in fatty acid oxidation and uptake, lipoprotein metabolism, and xenobiotic metabolism and transport) involved in lipid dysregulation and xenobiotic disposition were found to be induced by DB79 through bioinformatic annotation using dual-omics data. The biomolecular underpinnings of decreased transgenerational fertility in zebrafish attributable to BAD exposure were elucidated and novel biomolecular targets in the adverse outcome pathway framework were identified. These results will inform future studies and facilitate the development of mitigation strategies.</p>},
	keywords = {Animals, Azo Compounds, Female, Fertility, Male, Pregnane X Receptor, Water Pollutants, Chemical, Zebrafish},
	issn = {1873-3336},
	doi = {10.1016/j.jhazmat.2024.135442},
	author = {Wang, Chao and Gu, Wen and Zhang, Shaoping and Li, Li and Kong, Jian and Zhi, Hong and Liu, Juan and Wang, Mengmeng and Miao, Ke and Li, Qi and Yu, Jie and Wang, Runming and He, Runming and Zhang, Shuyi and Deng, Fuchang and Duan, Shuling and Zhang, Qiannan and Liu, Zhenming and Yang, Hui and Jia, Xudong and Peng, Hui and Tang, Song}
}
@article {130,
	title = {Toxicokinetics of Brominated Azo Dyes in the Early Life Stages of Zebrafish () Is Prone to Aromatic Substituent Changes.},
	journal = {Environ Sci Technol},
	volume = {54},
	year = {2020},
	month = {2020 04 07},
	pages = {4421-4431},
	abstract = {<p>Brominated azo dyes (BADs) have been identified as predominant indoor brominated pollutants in daycare dust; thus, their potential health risk to children is of concern. However, the toxicities of BADs remain elusive. In this study, the toxicokinetics of two predominant BADs, Disperse Blue 373 (DB373) and Disperse Violet 93 (DV93), and their suspect metabolite 2-bromo-4,6-dinitroaniline (BDNA) was investigated in embryos of zebrafish (). The bioconcentration factor of DV93 at 120 hpf is 6.2-fold lower than that of DB373. The nontarget analysis revealed distinct metabolism routes between DB373 and DV93 by reducing nitro groups to nitroso (DB373) or amine (DV93), despite their similar structures. NAD(P)H quinone oxidoreductase 1 (NQO1) and pyruvate dehydrogenase were predicted as the enzymes responsible for the reduction of DB373 and DV93 by correlating time courses of the metabolites and enzyme development. Further in vitro recombinant enzyme and in vivo inhibition results validated NQO1 as the enzyme specifically reducing DB373, but not DV93. Global proteome profiling revealed that the expression levels of proteins from the "apoptosis-induced DNA fragmentation" pathway were significantly upregulated by all three BADs, supporting the bioactivation of BADs to mutagenic aromatic amines. This study discovered the bioactivation of BADs via distinct eukaryotic enzymes, implying their potential health risks.</p>},
	keywords = {Animals, Azo Compounds, Child, Dust, Embryo, Nonmammalian, Humans, Mutagens, Toxicokinetics, Zebrafish},
	issn = {1520-5851},
	doi = {10.1021/acs.est.9b07178},
	author = {Han, Jiajun and Yang, Diwen and Hall, David Ross and Liu, Jiabao and Sun, Jianxian and Gu, Wen and Tang, Song and Alharbi, Hattan A and Jones, Paul D and Krause, Henry M and Peng, Hui}
}
