@article {121,
	title = {Molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among Escherichia coli isolates isolated from Wenyu River, China.},
	journal = {Chemosphere},
	volume = {87},
	year = {2012},
	month = {2012 Mar},
	pages = {37-42},
	abstract = {<p>Increasing antibacterial resistance and pathogenicity in the environment is of growing concern due to its potential human risk. In the present study, 236 Escherichia coli isolates were collected from Wenyu River in China on drugless (48 isolates) and quinolone-containing plates (189 isolates). Their minimum inhibitory concentrations (MICs) were determined ranging from 0.125 μg mL(-1) to 128 μg mL(-1). Mutation points related to fluoroquinolone resistance were observed at S83 to L and D87 to N or Y in the GyrA subunit and S80 to R or I and E84 to G in the ParC subunit. Generally, MICs of LEV and GAT are dependent on the patterns of these mutation points. The profile with three mutation points was related to LEV-resistant E. coli isolates, and the (S83L, D87N+S80I) mutation profile was most prevalent (65.7\%) in LEV-resistant isolates, while a large proportion of isolates, even those with three mutation points, were susceptive to GAT. The incidence of virulence factors in LEV-resistant isolates (44.7\%, 59/132) was much higher than in nonresistant isolates (23.1\%, 24/104) (χ(2)=11.925, 1{\textdegree} of freedom, p\&lt;0.001) indicating that fluoroquinolone-resistant E. coli would pose a potential risk. A similar distribution was also found in isolates resistant to GAT (χ(2)=7.843, 1{\textdegree} of freedom, p=0.0079).</p>},
	keywords = {Anti-Bacterial Agents, China, Drug Resistance, Bacterial, Escherichia coli, Fluoroquinolones, Rivers, Water Pollutants, Chemical},
	issn = {1879-1298},
	doi = {10.1016/j.chemosphere.2011.11.050},
	author = {Sun, Jianxian and Hu, Jianying and Peng, Hui and Shi, Jiachen and Dong, Zhaomin}
}
