Title | Signal Amplification for Imaging Mass Cytometry. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Rana, R, Gómez-Biagi, RF, Bassan, J, Nitz, M |
Journal | Bioconjug Chem |
Volume | 30 |
Issue | 11 |
Pagination | 2805-2810 |
Date Published | 2019 11 20 |
ISSN | 1520-4812 |
Keywords | Alkaline Phosphatase, Animals, Carbonic Anhydrase IX, Colonic Neoplasms, Humans, Image Cytometry, Indolequinones, Mice, Tumor Cells, Cultured, Xenograft Model Antitumor Assays |
Abstract | An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry. Upon phosphate hydrolysis, a quinone methide is released which covalently labels local nucleophiles. This strategy is a useful complement to heavy isotope antibody conjugates as it facilitates signal amplification for low-abundance biomarker detection. The workflow is conveniently integrated with standard IMC antibody staining to allow multiparametric antigen detection. |
DOI | 10.1021/acs.bioconjchem.9b00559 |
Alternate Journal | Bioconjug Chem |
PubMed ID | 31693335 |