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Signal Amplification for Imaging Mass Cytometry.

TitleSignal Amplification for Imaging Mass Cytometry.
Publication TypeJournal Article
Year of Publication2019
AuthorsRana, R, Gómez-Biagi, RF, Bassan, J, Nitz, M
JournalBioconjug Chem
Volume30
Issue11
Pagination2805-2810
Date Published2019 11 20
ISSN1520-4812
KeywordsAlkaline Phosphatase, Animals, Carbonic Anhydrase IX, Colonic Neoplasms, Humans, Image Cytometry, Indolequinones, Mice, Tumor Cells, Cultured, Xenograft Model Antitumor Assays
Abstract

An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry. Upon phosphate hydrolysis, a quinone methide is released which covalently labels local nucleophiles. This strategy is a useful complement to heavy isotope antibody conjugates as it facilitates signal amplification for low-abundance biomarker detection. The workflow is conveniently integrated with standard IMC antibody staining to allow multiparametric antigen detection.

DOI10.1021/acs.bioconjchem.9b00559
Alternate JournalBioconjug Chem
PubMed ID31693335