
@article {128,
	title = {DNA directed damage using a brominated DAPI derivative.},
	journal = {Chem Commun (Camb)},
	volume = {55},
	year = {2019},
	month = {2019 Aug 15},
	pages = {9971-9974},
	abstract = {<p>Photodynamic therapy (PDT) is a clinically approved cancer treatment that uses light, oxygen and a photosensitizer to produce localized reactive oxygen species (ROS). Due to the short lifetime of ROS, the location of the photosensitizer in the cell is believed to be the key determinant governing the outcome of PDT. To explore the effect of direct association between a photosensitizer and DNA a well know DNA-binding dye, DAPI, was converted into a photosensitizer. Br-DAPI - unlike native DAPI - upon irradiation produces ROS. We demonstrate that the ROS are only effective in inducing dsDNA breaks when Br-DAPI is bound to DNA. In cancer cells (A549) Br-DAPI causes rapid light dependent cell death. This work supports the design of photosensitizers which bind with high affinity to the DNA of target cells for potentially more effective PDT.</p>},
	keywords = {A549 Cells, Apoptosis, Bromine, Cell Survival, DNA, DNA Damage, Fluorescent Dyes, Humans, Indoles, Light, Photochemotherapy, Photosensitizing Agents, Proof of Concept Study, Reactive Oxygen Species},
	issn = {1364-548X},
	doi = {10.1039/c9cc03942b},
	author = {Digby, Elyse M and Rana, Rahul and Nitz, Mark and Beharry, Andrew A}
}
