@article {126, title = {Signal Amplification for Imaging Mass Cytometry.}, journal = {Bioconjug Chem}, volume = {30}, year = {2019}, month = {2019 11 20}, pages = {2805-2810}, abstract = {

An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry. Upon phosphate hydrolysis, a quinone methide is released which covalently labels local nucleophiles. This strategy is a useful complement to heavy isotope antibody conjugates as it facilitates signal amplification for low-abundance biomarker detection. The workflow is conveniently integrated with standard IMC antibody staining to allow multiparametric antigen detection.

}, keywords = {Alkaline Phosphatase, Animals, Carbonic Anhydrase IX, Colonic Neoplasms, Humans, Image Cytometry, Indolequinones, Mice, Tumor Cells, Cultured, Xenograft Model Antitumor Assays}, issn = {1520-4812}, doi = {10.1021/acs.bioconjchem.9b00559}, author = {Rana, Rahul and G{\'o}mez-Biagi, Rodolfo F and Bassan, Jay and Nitz, Mark} }